Journal: Frontiers in Pharmacology

Article Title: Molecular Mechanisms Underlying the Inhibition of Proliferation and Differentiation by Florfenicol in P19 Stem Cells: Transcriptome Analysis

doi: 10.3389/fphar.2022.779664

Figure Lengend Snippet: FLO inhibits the proliferation and pluripotency of P19SCs. (A) P19SC viability in response to FLO-treatment as detected by CCK-8 method. The results are expressed as the average cell viabilities from three independent experiments. (B) Effect of different doses of FLO on LDH release of P19SCs when exposed for 48 h; ns , not significant. (C) Immunoblot analysis of multiple doses of FLO on protein levels of pluripotent markers (Sox2 and Oct4) in P19SCs treated for 48 h.

Article Snippet: The medium was removed, and 100 μL PBS containing 10 μL CCK-8 solution (TransGen Biotech, China) was added into each well and maintained at 37°C.

Techniques: CCK-8 Assay, Western Blot

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: circ_0119412 downregulation suppressed cervical cancer cell malignant behaviors and tumor growth in animal models. (A) The efficiency of circ_0119412 overexpression was checked by RT‐qPCR. (B) Cell proliferation affected by circ_0119412 overexpression was assessed by CCK‐8 assay. (C) Cell migration affected by circ_0119412 overexpression was assessed by transwell assay. (D) Cell adhesion affected by circ_0119412 overexpression was assessed by MTT assay. (E) The role of circ_0119412 in vivo was assessed by animal study. *p < 0.05, **p < 0.001 relative to oe‐NC

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Over Expression, Quantitative RT-PCR, CCK-8 Assay, Migration, Transwell Assay, MTT Assay, In Vivo

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: miR‐217 upregulation reversed the effects caused by circ_0119412 overexpression. (A) The expression of miR‐217 in Hela and SiHa cells transfected with oe‐circ, miR‐217 mimic, or oe‐circ+miR‐217 mimic was checked by RT‐qPCR. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were monitored by CCK‐8, transwell, or MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05, && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐circ+mimic

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Over Expression, Expressing, Transfection, Quantitative RT-PCR, Migration, CCK-8 Assay, MTT Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR‐217 to upregulate anterior gradient 2

doi: 10.1002/jcla.24236

Figure Lengend Snippet: miR‐217 inhibited cervical cancer cell malignant behaviors by degrading AGR2. (A) The expression of AGR2 protein in Hela and SiHa cells transfected with oe‐AGR2, miR‐217 mimic, or oe‐AGR2+miR‐217 mimic was measured by western blot. In these transfected cells, (B‐D) cell proliferation, migration, and adhesion were investigated by CCK‐8, transwell, and MTT assay, respectively, **p < 0.001 relative to oe‐NC; & p < 0.05 and && p < 0.001 relative to mimic‐NC; # p < 0.05, ## p < 0.001 relative to oe‐AGR2+mimic

Article Snippet: CCK‐8 reagent (Cwbio) was used to incubate cells in different wells at the indicated time points (24, 48, 72 or 96 h post‐seeding).

Techniques: Expressing, Transfection, Western Blot, Migration, CCK-8 Assay, MTT Assay

Journal: Annals of Translational Medicine

Article Title: TGR5 overexpression mediated by the inhibition of transcription factor SOX9 protects against hypoxia-/reoxygenation-induced injury in hippocampal neurons by activating Nrf2 / HO-1 signaling

doi: 10.21037/atm-22-5225

Figure Lengend Snippet: The elevation of TGR5 contributes to HT22 cell viability after H/R. (A) RT-qPCR and (B) western blots were used to determine TGR5 expression in the HT22 cells in the absence or presence of H/R treatment. (C) RT-qPCR and (D) western blot analyses of the overexpression efficacy of pcDNA3.1-TGR5 plasmid. The viability of the H/R-treated HT22 cells was assessed by (E) CCK-8 assays. (F) LDH release was measured by a LDH assay kit. *, P<0.05; **, P<0.01; ***, P<0.001. H/R, hypoxia/reoxygenation; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LDH, lactate dehydrogenase.

Article Snippet: The cells were cultivated for another 2 h at 37 ℃ after the addition of 10 µL of CCK-8 solution (Beijing Transgen Biotech Co., Ltd.).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Over Expression, Plasmid Preparation, CCK-8 Assay, Lactate Dehydrogenase Assay, Real-time Polymerase Chain Reaction

Journal: Annals of Translational Medicine

Article Title: TGR5 overexpression mediated by the inhibition of transcription factor SOX9 protects against hypoxia-/reoxygenation-induced injury in hippocampal neurons by activating Nrf2 / HO-1 signaling

doi: 10.21037/atm-22-5225

Figure Lengend Snippet: The upregulation of SOX9 reverses the inhibitory role of TGR5 in H/R-triggered HT22 cell injury. The viability of H/R-treated HT22 cells was assessed by (A) CCK-8 assays; (B) LDH release was measured by the LDH assay kit. Corresponding kits were used to examine the levels of SOD, GSH-Px, and MDA. The apoptotic ability of the H/R-treated HT22 cells was evaluated by (C) TUNEL assays (magnification, 200×). (D) IF staining was used to detect cyto-c release (magnification, 200×). (E) Western blots were used analyze the protein levels of Bcl-2, Bax, cleaved caspase-3, and cleaved-PARP. *, P<0.05; **, P<0.01; ***, P<0.001. H/R, hypoxia/reoxygenation; NC, negative control; CCK-8, Cell Counting Kit-8; LDH, lactate dehydrogenase; SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; MDA, malondialdehyde; C-Caspase3, cleaved caspase3; C-PARP, cleaved-PARP.

Article Snippet: The cells were cultivated for another 2 h at 37 ℃ after the addition of 10 µL of CCK-8 solution (Beijing Transgen Biotech Co., Ltd.).

Techniques: CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Staining, Western Blot, Negative Control, Cell Counting